Muscle Differentiation
Overview
We accept the following cell types for differentiation into skeletal muscle myoblasts:
- Human induced pluripotent stem cells (iPSCs)
- Human embryonic stem cells (ESCs)
We are happy to advise on obtaining an appropriate cell line for your project.
See pricing and service details, see below.
To request a quote or discuss your project with us, please use our service request form.
Have another idea? Custom projects can be arranged by consultation; please contact us.
Rates
To place an order, please use our service request form. The HSCI iPS Core offers the following differentiation services (rates differ according to Harvard affiliation and for-profit/non-profit status; all rates are subject to change):
| Differentiation | INTERNAL BASE RATES FOR HARVARD AND HMS FACULTY* | HSCI AFFILIATES | EXTERNAL NON-PROFIT RESEARCH | EXTERNAL COMMERCIAL (FOR-PROFIT) |
|---|---|---|---|---|
Module 1: Skeletal Muscle Differentiation (1 sample) | $1,781 | $2,048 | $2,547 | $3,562 |
*"Harvard and HMS Faculty" includes two groups of users: 1) all Harvard faculty and researchers using a Harvard University 33-digit billing code, and 2) HMS Quad A and Quad B faculty members.
Service Details
Differentiation Module 1: hPSC differentiation into skeletal muscle
In this module, which takes about 8-12 weeks to complete, the iPS Core will differentiate a human pluripotent stem cell (hPSC) line into functional myoblasts.
Service steps include an initial thawing/passaging of the hPSC line of interest, 30 days of stepwise differentiation, filtration of early myoblasts, and a final expansion.
This module delivers a cryopreserved stock of the early myoblasts (at p0) and a cryopreserved stock of expanded myoblasts (at p1). The expanded myoblasts are optimal for secondary differentiation into myofibers.
