Cell Expansion Services
Overview
We accept the following cell types for expansion services:
- Human induced pluripotent stem cells (iPSCs)
- Human embryonic stem cells (ESCs)
Available services include:
- Transition from feeder to feeder-free culture
- Stock expansion from 1 vial into 5 or 20 vials (or custom number)
Sub-cloning
For pricing and service details, see below.
To request a quote or discuss your project with us, please use our service request form.
Have another idea? Custom projects can be arranged by consultation; please contact us.
Rates
To place an order, please use our service request form. The HSCI iPS Core offers the following expansion services (rates differ according to Harvard affiliation and for-profit/non-profit status; all rates are subject to change):
| EXPANSION SERVICES | INTERNAL BASE RATES FOR HARVARD AND HMS FACULTY* | HSCI AFFILIATE INSTIUTIONS | EXTERNAL NON-PROFIT | EXTERNAL COMMERCIAL (FOR-PROFIT) |
|---|---|---|---|---|
Module 1: Transition from feeder to feeder-free culture | $1,514 | $1,742 | $2,166 | $3,029 |
Module 2: Expansion and freezing from 1 to 5 vials | $823 | $947 | $1,177 | $1,646 |
Module 3: Expansion and freezing from 1 to 20 vials | $1,709 | $1,965 | $2,443 | $3,417 |
Module 4: Sub-cloning | $2,258 | $2,597 | $3,229 | $4,516 |
Module 5: Custom Expansion | Custom | |||
*"Harvard and HMS Faculty" includes two groups of users: 1) all Harvard faculty and researchers using a Harvard University 33-digit billing code, and 2) HMS Quad A and Quad B faculty members.
Service Details
Module 1: Transition from feeder to feeder-free culture (from 1 cryovial on MEFs to 5 cryovials on feeder-free culture)
One cryovial of a human pluripotent stem cell line is thawed onto feeder culture conditions. The cells are passaged once on feeder culture conditions before the transition to feeder-free. The cells are then expanded to 2x10cm plates and frozen into 6 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma testing. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, StemFlex, mTeSR Plus or other media if needed).
Module 2: Expansion and freezing (from 1 vial into 5 vials)
One cryovial of a human pluripotent stem cell line is thawed onto feeder free culture conditions. The cells are expanded to 2x10cm plates and frozen into 6 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma testing. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, StemFlex, mTeSR Plus or other media if needed).
Module 3: Expansion and freezing (from 1 vial into 20 vials)
One cryovial of a human pluripotent stem cell line is thawed onto feeder free culture conditions. The cells are expanded to 7x10cm plates and frozen down into 21 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma testing. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, StemFlex, mTeSR Plus or other media if needed).