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Expansion Services

To place an order, please use the service request form. The HSCI iPS Core offers the following expansion services (rates differ according to Harvard affiliation and for-profit/non-profit status and are subject to change):

 

Internal Rates for Harvard and HMS Faculty+*

Expansion Services

Module 1: Transition from feeder to feeder-free culture           $1,683          
Module 2: Expansion and freezing from 1 to 5 vials $915
Module 3: Expansion and freezing from 1 to 20 vials $1,898
Module 4: Sub-cloning $2,509
Characterization Service Modules 1 iPS line/sample 2 iPS lines/sample 3 iPS lines/sample 4 iPS lines/sample
Module 3: ICC, EB formation, gene expression $1,913 $2,245 $2,663 $3,068
Module 4: Karyotyping $784 $1,364 $1,943 $2,522
Module 5: Karyotyping + STR (DNA fingerprinting) *NEW* $1,014 $1,824 $2,633 $3,442
Module 6: Karyostat *NEW* $718 $1,201 $1,684 $2,168
Module 7: StemGenomics ddPCR iCS-digital Pluri 24 probes *NEW* $477 $720 $963 $1,205
Module 8: ddPCR for BCL211 $296 $366 $437 $507
Module 9: Sendai virus elimination testing $307 $408 $481 $554
Module 10: RT-PCR and sequencing *NEW* $338 $405 $473

$540


+ "Harvard and HMS Faculty" includes two groups of users: 1) all Harvard faculty and researchers using a Harvard University 33-digit billing code and 2) HMS Quad A and Quad B faculty members.
* HSCI Affiliate Faculty rates are no longer applicable as of July 1, 2023.

 

Rates for Non-Profit Institutions

Expansion Services

Module 1: Transition from feeder to feeder-free culture           $2,406         
Module 2: Expansion and freezing from 1 to 5 vials $1,308
Module 3: Expansion and freezing from 1 to 20 vials $2,715
Module 4: Sub-cloning $3,588
Characterization Service Modules 1 iPS line/sample 2 iPS lines/sample 3 iPS lines/sample 4 iPS lines/sample
Module 3: ICC, EB formation, gene expression $2,736 $3,211 $3,808 $4,388
Module 4: Karyotyping $915 $1,743 $2,572 $3,400
Module 5: Karyotyping + STR (DNA fingerprinting) *NEW* $1,145 $2,302 $3,460

$4,617

Module 6: Karyostat *NEW* $841 $1,532 $2,223 $2,914
Module 7: StemGenomics ddPCR iCS-digital Pluri 24 probes *NEW* $597 $944 $1,290 $1,637
Module 8: ddPCR for BCL2l1 $423 $524 $625 $725
Module 9: Sendai virus elimination testing $438 $584 $688 $792
Module 10: RT-PCR and sequencing *NEW* $483 $579 $676 $772
  

Rates for For-Profit Institutions

Expansion Services

Module 1: Transition from feeder to feeder-free culture           $4,066         
Module 2: Expansion and freezing from 1 to 5 vials $2,210
Module 3: Expansion and freezing from 1 to 20 vials $4,588
Module 4: Sub-cloning $6,064
Characterization Service Modules 1 iPS line/sample 2 iPS lines/sample 3 iPS lines/sample 4 iPS lines/sample
Module 3: ICC, EB formation, gene expression $4,624 $5,426 $6,436 $7,416
Module 4: Karyotyping $1,215 $2,615 $4,015 $5,415
Module 5: Karyotyping + STR (DNA fingerprinting) *NEW* $1,445 $3,401 $5,357 $7,313
Module 6: Karyostat *NEW* $1,123 $2,291 $3,459 $4,627
Module 7: StemGenomics ddPCR iCS-digital Pluri 24 probes *NEW* $870 $1,457 $2,043 $2,629
Module 8: ddPCR for BCL2l1 $715 $885 $1,056 $1,226
Module 9: Sendai virus elimination testing $741 $987 $1,163 $1,339
Module 10: RT-PCR and sequencing *NEW* $816 $979 $1,142 $1,305

 

Module 1: Transition from feeder to feeder-free culture (from 1 vial on MEFs to 5 cryovials on feeder-free culture)

One cryovial of human pluripotent stem cell line is thawed onto feeder culture condition. The cells are split once on feeder-culture condition before the transition to feeder-free. The cells are then expanded to 2x10cm plates and frozen down into 6 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma test. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, Stemflex, mTeSr+ or other media if needed).

 

Module 2: Expansion and freezing (from 1 to 5 vials)

One cryovial of human pluripotent stem cell line is thawed onto feeder free culture condition. The cells are then expanded to 2x10cm plates and frozen down into 6 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma test. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, Stemflex, mTeSr+ or other media if needed).

 

Module 3: Expansion and freezing (from 1 to 20 vials)

One cryovial of Human pluripotent stem cell line is thawed onto feeder free culture condition. The cells are then expanded to 7x10cm plates and frozen down into 21 cryovials. One cryovial is thawed to test the recovery post-thawing and for mycoplasma test. Different feeder-free culture conditions can be used (Coating: Cultrex, Vitronectin, Laminin 521. Media: mTeSR1, Stemflex, mTeSr+ or other media if needed).

 

Module 4: Sub-cloning

[description forthcoming]