Characterization Services
To place an order, please use the service request form. The HSCI iPS Core offers the following characterization services (rates differ according to Harvard affiliation and for-profit/non-profit status; all rates are subject to change):
INTERNAL BASE RATES FOR HARVARD AND HMS FACULTY*
| CHARACTERIZATION SERVICES (Price based on the number of iPS lines characterized per sample) | Price for 1 iPS line/ sample | Price for 2 iPS lines/sample | Price for 3 iPS lines/sample | Price for 4 iPS line/sample |
|---|---|---|---|---|
Module 3: ICC, EB Formation, Gene expression | $1,721 | $2,021 | $2,397 | $2,762 |
Module 4: Karyotyping | $784 | $1,383 | $1,983 | $2,582 |
Module 5: Karyotyping +STR (DNA fingerprinting) | $1,034 | $1,883 | $2,733 | $3,582 |
Module 6a: GSA SNP array | $216 | $310 | $403 | $496 |
Module 6b: GDA SNP array | $342 | $561 | $779 | $998 |
Module 7: StemGenomics ddPCR iCS-digital Pluri 28 probes | $450 | $688 | $926 | $1,165 |
Module 8: ddPCR for BCL2L1 | $266 | $330 | $393 | $457 |
Module 9: Sendai virus elimination testing | $276 | $367 | $433 | $499 |
Module 10: RT-PCR and Sequencing | $304 | $365 | $425 | $486 |
Module 11: Immunostaining | $240 | $287 | $335 | $383 |
*"HARVARD AND HMS FACULTY" includes two groups of users: (1) All Harvard faculty and researchers using HU 33-digit billing code, and (2) HMS Quad A and Quad B Faculty Members
HSCI AFFILIATE INSTITUTIONS
| CHARACTERIZATION SERVICES (Price based on the number of iPS lines characterized per sample) | Price for 1 iPS line/ sample | Price for 2 iPS lines/sample | Price for 3 iPS lines/sample | Price for 4 iPS line/sample |
|---|---|---|---|---|
Module 3: ICC, EB Formation, Gene expression | $1,979 | $2,324 | $2,756 | $3,176 |
Module 4: Karyotyping | $825 | $1,438 | $2,050 | $2,663 |
Module 5: Karyotyping +STR (DNA fingerprinting) | $1,075 | $1,938 | $2,800 | $3,663 |
Module 6a: GSA SNP array | $239 | $336 | $433 | $530 |
Module 6b: GDA SNP array | $364 | $587 | $809 | $1,032 |
Module 7: StemGenomics ddPCR iCS-digital Pluri 28 probes | $487 | $731 | $975 | $1,219 |
Module 8: ddPCR for BCL2L1 | $306 | $379 | $452 | $525 |
Module 9: Sendai virus elimination testing | $317 | $423 | $498 | $573 |
Module 10: RT-PCR and Sequencing | $350 | $419 | $489 | $559 |
Module 11: Immunostaining | $276 | $331 | $386 | $441 |
EXTERNAL NON-PROFIT INSTITUTIONS
| CHARACTERIZATION SERVICES (Price based on the number of iPS lines characterized per sample) | Price for 1 iPS line/ sample | Price for 2 iPS lines/sample | Price for 3 iPS lines/sample | Price for 4 iPS line/sample |
|---|---|---|---|---|
Module 3: ICC, EB Formation, Gene expression | $2,461 | $2,890 | $3,427 | $3,949 |
Module 4: Karyotyping | $902 | $1,539 | $2,177 | $2,815 |
Module 5: Karyotyping +STR (DNA fingerprinting) | $1,152 | $2,039 | $2,927 | $3,815 |
Module 6a: GSA SNP array | $280 | $384 | $488 | $593 |
Module 6b: GDA SNP array | $405 | $635 | $865 | $1,094 |
Module 7: StemGenomics ddPCR iCS-digital Pluri 28 probes | $557 | $812 | $1,067 | $1,321 |
Module 8: ddPCR for BCL2L1 | $381 | $472 | $562 | $653 |
Module 9: Sendai virus elimination testing | $395 | $525 | $619 | $713 |
Module 10: RT-PCR and Sequencing | $435 | $522 | $608 | $695 |
Module 11: Immunostaining | $343 | $411 | $479 | $548 |
EXTERNAL COMMERCIAL (FOR-PROFIT)
| CHARACTERIZATION SERVICES (Price based on the number of iPS lines characterized per sample) | Price for 1 iPS line/ sample | Price for 2 iPS lines/sample | Price for 3 iPS lines/sample | Price for 4 iPS line/sample |
|---|---|---|---|---|
Module 3: ICC, EB Formation, Gene expression | $3,442 | $4,042 | $4,794 | $5,523 |
Module 4: Karyotyping | $1,058 | $1,746 | $2,256 | $3,124 |
Module 5: Karyotyping +STR (DNA fingerprinting) | $1,308 | $2,246 | $3,185 | $4,124 |
Module 6a: GSA SNP array | $364 | $483 | $602 | $720 |
Module 6b: GDA SNP array | $490 | $734 | $978 | $1,222 |
Module 7: StemGenomics ddPCR iCS-digital Pluri 28 probes | $699 | $976 | $1,253 | $1,529 |
Module 8: ddPCR for BCL2L1 | $533 | $659 | $786 | $913 |
Module 9: Sendai virus elimination testing | $552 | $735 | $866 | $997 |
Module 10: RT-PCR and Sequencing | $608 | $729 | $851 | $972 |
Module 11: Immunostaining | $479 | $575 | $670 | $766 |
Characterization Services
Our current characterization services are only offered for iPS lines derived at the Core. Once iPS lines are established, we will discuss with you which module(s) of characterization you will choose to have the Core perform. The workflow is optimized for proceeding without having to restart the cultures.
Module 3: ICC, EB formation, and Gene Expression
This service, which takes about 1 month to complete, includes:
- ICC for Oct4, Nanog, TRA-1-60, SSEA4
- RNA extraction, cDNA synthesis and qRT-PCR for endogenous Oct4, Nanog, endogenous Sox2, Rex1, hTERT, Dnmt3b
- EB formation (15 days differentiation), RNA extraction, cDNA synthesis and qRT-PCR for 2 mesoderm markers, 2 ectoderm markers and 2 endoderm markers
The data will be sent to the investigator in PowerPoint by email.
Module 4: Karyotyping
This service, which takes about 1 month to complete, includes:
- Expanding the iPS line to a T25 flask
- Mycoplasma testing
- Shipping the line to Cell Line Genetics for karyotyping
The reports will be sent to the investigator by e-mail two to three weeks later.
Module 5: Karyotyping + STR Analysis (DNA fingerprinting)
Module 7: StemGenomics ddPCR iCS-digital Pluri 28 probes
Module 8: ddPCR for BCL2L1
Module 9: Sendai virus elimination testing