Genome Editing Services

To place an order, please use the service request form. The HSCI iPS Core offers the following genome editing services (rates differ according to Harvard affiliation and profit/non-profit status)*:

Genome editing services
TALEN technology modules Rates for Harvard and HSCI Faculty+ Rates for Non-profit Institutions
Module 1: Construction/Testing TALEN vectors $3,300/TALEN pair $3,700/TALEN pair
Module 2: TALEN-mediated gene knockout $13,800/gene/hPSC line $17,400/gene/hPSC line
Module 3: TALEN-mediated gene mutation introduction and/or repair $19,500/mutation/hPSC line $23,200/mutation/hPSC line
CRISPR technology modules
Module 1: Construction and testing of CRISPR vectors $1,970/CRISPR vector $2,140/CRISPR vector
Module 2: CRISPR/Cas9-mediated gene knock-out $13,500/gene/hPSC line $17,400/gene/hPSC line
Module 3: CRISPR/Cas9-mediated gene mutation introduction and/or repair $15,200/mutation/hPSC line $19,100/mutation/hPSC line

+Note: "Harvard and HSCI Faculty" includes two groups of users: (1) All Harvard faculty and researchers using HU 33-digit charging code and (2) faculty with HSCI appointments.

*Prices listed reflect the price for non-profit institutions only. The HSCI iPS Core facility is pleased to offer these services to for-profit institutions. PleaseĀ contact us for a list of prices if you are a for-profit institution.

Process Guideline

TALEN Module 1: Construction/Testing of TALEN vectors

This module, which takes 2 to 3 weeks to complete, comprises consults with the investigator to discuss which gene locus needs to be targeted and what the best TALEN design and targeting strategy would be to generate the edited human pluripotent stem cell (hPSC) line of interest.

After (in silico) design of the TALEN arrays, TALEN plasmid pairs are constructed from our TALEN 4/3-mer library targeting DNA sequences in genomic loci as specified by the investigator. Upon request, the TALEN plasmid pairs can be validated for nuclease activity with a genomic cleavage assay.

TALEN Module 2: TALEN-mediated gene knockout

This service, which takes about 6 months to complete, includes:

  • hPSC expansion/adaptation to single cell passaging
  • Mycoplasma testing
  • Electroporation of TALEN pair into 1 hPSC line
  • FACS sorting (pre-selection) of electroporated hPSCs
  • Clonal expansion of targeted line
  • PCR screening of targeted clones, followed by DNA Sanger sequencing of mutant clones to verify correct targeting
  • Expansion of mutant clones
  • Mycoplasma testing
  • Freezing (6 vials per line)

TALEN Module 3: TALEN-mediated gene mutation introduction and/or repair

This service, which takes 6 to 7 months to complete, includes:

  • hPSC expansion/adaptation to single cell passaging
  • Mycoplasma testing
  • Electroporation of 1 pair of TALEns and a single stranded oligodinucleotide (ssODN) containing wildtype or mutated DNA sequence into 1 hPSC line
  • FACS sorting (pre-selection) electroporated hPSCs
  • Clonal expansion of targeted line
  • PCR/RE digest screening of targeted clones, followed by DNA Sanger sequencing to verify correct targeting and introduction of gene mutation/repair
  • Expansion of mutant clones
  • Mycoplasma testing
  • Freezing (6 vials per line)

For introducing or repairing mutations in hPSC lines in this module, we will design and have synthesized an ssODN containing the mutated or corrected DNA sequence, respectively.

CRISPR Module 1: Construction/Testing CRISPR vectors

This module, which takes about 2 weeks to complete, comprises consults with the investigator to discuss what the best CRISPR design and targeting strategy would be to generate an edited human pluripotent stem cell (hPSC) line with the investigatorsā€™ gene of interest targeted.

The module delivers customized construction of a CRISPR guide RNA plasmid containing a synthetic guide RNA complementary to the DNA sequence in the genomic site to be targeted.

Upon request the CRISPR guide RNA plasmid can be validated for nuclease efficiency by co-transfection of the CRISPR guide RNA plasmid and a Cas9-GFP plasmid using a genomic cleavage assay.

CRISPR Module 2: CRISPR/Cas9-mediated gene knock-out

This service, which takes about 6 months to complete, includes:

  • hPSC expansion/adaptation to single cell passaging
  • Mycoplasma testing
  • Electroporation of CRIPSR guide RNA and Cas9-GFP plasmids into 1 hPSC line
  • FACS sorting (pre-selection) of electroporated hPSCs
  • Clonal expansion of targeted line
  • PCR screening of targeted clones, followed by DNA Sanger sequencing of mutant clones to verify correct targeting
  • Expansion of mutant clones
  • Mycoplasma testing
  • Freezing (6 vials per line)

CRISPR Module 3: CRISPR/Cas9-mediated gene mutation introduction and/or repair

This service, which takes 6 to 7 months to complete, includes:

  • hPSC expansion/adaptation to single cell passaging
  • Mycoplasma testing
  • Electroporation of a CRISPR guide RNA, Cas9-GFP plasmid and a single stranded oligodinucleotide (ssODN) containing wildtype or mutated DNA sequence into 1 hPSC line
  • FACS sorting (pre-selection) electroporated hPSCs
  • Clonal expansion of targeted line
  • PCR/RE digest screening of targeted clones, followed by DNA Sanger sequencing to verify correct targeting and introduction of gene mutation/repair
  • Expansion of mutant clones
  • Mycoplasma testing
  • Freezing (6 vials per line)

For introducing or repairing mutations in hPSC lines in this moduel, we will design and have synthesized an ssODN containing the mutated or corrected DNA sequence, respectively.