The Most Experienced iPS Cell Team in the Nation

In July 2008, the Harvard Stem Cell Institute iPS Core Facility was created to accelerate research in the stem cell field by facilitating the derivation and distribution of induced pluripotent stem (iPS) cell lines. Disease-specific iPS cell lines provide us with an opportunity to study the mechanisms of disease and ultimately to develop new treatments.

In recent years, we have refined the iPS Core to better meet investigator needs. The iPS Core now has a fee-for-service business model to provide investigators with customized iPS derivation and genome editing based on the latest cutting-edge stem cell technologies.

  • We offer three non-integrating technologies (Sendai virus, miRNA and modified mRNA, and episomal vector) for reprogramming of a variety of somatic cell types.
  • In 2013, a new service was launched, offering the generation of genome engineered hESC or hiPS lines. We currently offer TALEN or CRISPR genome editing technologies to generate knock-out or knock-in lines for repair or introduction of mutations.
  • The iPS Core also serves as a repository for Harvard hESC lines and both control and disease-specific hiPS cell lines.

All of these services are available to the global research community.

Announcements

New Episomal Vector Technology and Quantity Discounts

April 16, 2014

The HSCI iPS Core's newest derivation service is reprogramming somatic cells using episomal vector technology. The service, which takes 2-3 months to complete costs $6,200 (2-5 colonies)/sample.

All derivation service costs can now be reduced through a quantity discount:

  • 5-9 samples - 10% off
  • 10-14 samples - 20% off
  • 15 or more samples - 25% off

For more information, please see ourĀ complete list of derivation services or contact Laurence Daheron.

New CRISPR Services

March 13, 2014

The HSCI iPS Core has added several new CRISPR technology modules to its genome editing services:

  • Construction/Testing CRISPR vectors - This module delivers customized construction of a CRISPR guide RNA plasmid containing a synthetic guide RNA complementary to the DNA sequence in the genomic site to be targeted.
  • CRISPR/Cas9-mediated gene knock-out - This service includes electroporation of CRIPSR guide RNA and Cas9-GFP plasmids into 1 hPSC line.
  • CRISPR/Cas9-mediated gene introduction and/or repair - This service includes electroporation of a CRISPR guide RNA, Cas9-GFP plasmid and a single stranded oligodinucleotide (ssODN) containing wildtype or mutated DNA sequence into 1 hPSC line.

For more information, please see our complete list of genome editing services or contact William Hendriks.