March 13, 2014
The HSCI iPS Core has added several new CRISPR technology modules to its genome editing services:
- Construction/Testing CRISPR vectors - This module delivers customized construction of a CRISPR guide RNA plasmid containing a synthetic guide RNA complementary to the DNA sequence in the genomic site to be targeted.
- CRISPR/Cas9-mediated gene knock-out - This service includes electroporation of CRIPSR guide RNA and Cas9-GFP plasmids into 1 hPSC line.
- CRISPR/Cas9-mediated gene introduction and/or repair - This service includes electroporation of a CRISPR guide RNA, Cas9-GFP plasmid and a single stranded oligodinucleotide (ssODN) containing wildtype or mutated DNA sequence into 1 hPSC line.