Registration remains open for "CRISPR Genome Editing," a four-day, hands on workshop sponsored by the Harvard Stem Cell Institute. Only a few seats remain, though, so register by the deadline of May 11, 2018. You will need to upload your CV and a letter of motivation to be considered for this workshop.
Registration is now closed (we are fully subscribed) for "Human iPSC Derivation and Culture," a three-day, hands-on workshop co-sponsored by HSCI and the UK Stem Cell Bank. Participants will learn current reprogramming techniques, and receive information about the following topics in iPSC technology:
Hands-on technical training in current reprogramming methodologies
Presentation of Sendai virus, episomal vector and mRNA reprogramming
The HSCI iPS Core has added several new CRISPR technology modules to its genome editing services:
Construction/Testing CRISPR vectors - This module delivers customized construction of a CRISPR guide RNA plasmid containing a synthetic guide RNA complementary to the DNA sequence in the genomic site to be targeted.
CRISPR/Cas9-mediated gene knock-out - This service includes electroporation of CRIPSR guide RNA and Cas9-GFP plasmids into 1 hPSC line.
CRISPR/Cas9-mediated gene introduction and/or repair - This service includes electroporation of a CRISPR guide RNA, Cas9-GFP plasmid and a single stranded oligodinucleotide (ssODN) containing wildtype or mutated DNA sequence into 1 hPSC line.