#  Gene Editing Services 

 



 ##  

  expand\_more  

 
  

 

## Overview

We accept the following **cell types** for gene editing:

- Human induced pluripotent stem cells (iPSCs)
- Human embryonic stem cells (ESCs)

We are happy to advise on obtaining an appropriate cell line for your project.

Our gene editing services include:

- CRISPR gene knock-outs
- CRISPR knock-ins
    - Introduce or repair a mutation/modify a SNP
    - Insert a short sequence <50 nt
- CRISPR-mediated transgene insertion
    - E.g. Gene reporter lines
- Selection cassette removal
    - Cre-LoxP, PiggyBac or FLP-FRT

See **pricing and service details**, see below.

To **request a quote** or **discuss your project with us**, please use our [service request form](https://ipscore.hsci.harvard.edu/service-request-form).

Have another idea? **Custom projects** can be arranged by consultation; please [contact us](/contact-us "Contact Us").

## Rates

To place an order, please use our [service request form](https://ipscore.hsci.harvard.edu/service-request-form). The HSCI iPS Core offers the following gene editing services (rates differ according to Harvard affiliation and for-profit/non-profit status; all rates are subject to change):

SortGENOME EDITING SERVICESINTERNAL BASE RATES FOR HARVARD & HMS FACULTY\*HSCI AFFILIATE INSTITUTIONSEXTERNAL NON-PROFIT INSTITUTIONS**Module 1: Background study and gRNA design**

$492

$566

$703

**Module 2: CRISPR-mediated KO relying on indel to create frameshift**

$10,879

$12,511

$15,557

**Module 3: CRISPR-mediated KI using ssODN**

$12,549

$14,431

$17,945

**Module 4: CRISPR-mediated transgene insertion with selection cassette**

$8,643

$9,939

$12,359

**Module 5: Selection cassette removal with Cre-LoxP or PiggyBac**

$7,081

$8,144

$10,126





*\* "Harvard and HMS Faculty" includes two groups of users: 1) all Harvard faculty and researchers using a Harvard University 33-digit billing code, and 2) HMS Quad A and Quad B faculty members.*

## Service Details

### CRISPR Module 1: Background Study and gRNA Design

In this module, which takes about **two weeks** to complete, the iPS Core consults with the investigator to develop a targeting strategy for the generation of an edited human pluripotent stem cell (hPSC) line.

This module delivers custom design of CRISPR guide RNAs complementary to the DNA sequence in the genomic site to be targeted.

### CRISPR Module 2: CRISPR-mediated KO relying on indel to create frameshift

In this module, which takes about **8-12 weeks** to complete, the iPS Core targets a human pluripotent stem cell (hPSC) line to **produce a frameshift mutation** in a protein-coding gene, usually targeted to trigger decay of the mRNA transcript.

Service steps include transfection of one hPSC line with CRISPR guide RNA and Cas9 (Neon system: 3 voltages), analysis and subcloning of the transfected cell pool, screening of 96 clones, analysis and expansion of clones with desired genotypes, cryopreservation, and QC consisting of thaw testing, mycoplasma testing and sequence confirmation of the edited locus.

This module delivers cryopreserved stocks for four clones with desired genotypes; typically two knock-out and two non-targeted control clones.

### CRISPR Module 3: CRISPR-mediated KI using ssODN

In this module, which takes about **8-12 weeks** to complete, the iPS Core targets an hPSC line to **modify a genomic sequence using a short template** encoded in a single-stranded oligo DNA nucleotide (ssODN).

Examples include modifying a single nucleotide polymorphism (SNP) or inserting a short sequence <50 nt in length.

Service steps include transfection of one hPSC line with CRISPR guide RNA, Cas9 and ssODN; analysis and subcloning of the transfected cell pool, screening of 96 clones, analysis and expansion of clones with desired genotypes, cryopreservation, and QC consisting of thaw testing, mycoplasma testing and sequence confirmation of the edited locus.

This module delivers cryopreserved stocks for four clones with desired genotypes; typically two knock-in and two non-targeted control clones.

### CRISPR Module 4: CRISPR-mediated transgene insertion with selection cassette

In this module, which takes about **8-12 weeks** to complete, the iPS Core targets an hPSC line to **insert a transgenic sequence >50 nt in length that includes a selection marker**. The investigator provides a plasmid that encodes the transgenic sequence and homology arms corresponding to the desired insertion site.

Examples include the generation of reporter lines.

Service steps include transfection of one hPSC line with CRISPR guide RNA, Cas9 and plasmid; antibiotic selection of the transfected cell pools, analysis and expansion of clones with desired transgene insertion, cryopreservation and sequence confirmation of the edited locus. If selection cassette removal (Module 5) is not chosen, service steps of Module 4 will also include thaw testing and mycoplasma testing.

This module delivers cryopreserved stocks for two clones with desired transgene insertion.

### CRISPR Module 5: Selection cassette removal with Cre-LoxP, PiggyBac or FLP-FRT

In this module, which takes about **4-6 weeks** to complete, the iPS Core removes a cassette from an hPSC line using Cre recombinase, PiggyBac transposase or FLP recombinase.

Service steps include transfection of one hPSC line; subcloning of the transfected cell pool, analysis and expansion of clones with the selection cassette removed, cryopreservation, and QC consisting of thaw testing, mycoplasma testing and sequence confirmation of the edited locus.

This module delivers cryopreserved stocks for two clones with desired transgene removal.