#  Muscle Differentiation 

 



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## Overview

We accept the following **cell types** for differentiation into **skeletal muscle myoblasts**:

- Human induced pluripotent stem cells (iPSCs)
- Human embryonic stem cells (ESCs)

We are happy to advise on obtaining an appropriate cell line for your project.

See **pricing and service details**, see below.

To **request a quote** or **discuss your project with us**, please use our [service request form](https://ipscore.hsci.harvard.edu/service-request-form).

Have another idea? **Custom projects** can be arranged by consultation; please [contact us](/contact-us "Contact Us").

## Rates

To place an order, please use our [service request form](https://ipscore.hsci.harvard.edu/service-request-form). The HSCI iPS Core offers the following differentiation services (rates differ according to Harvard affiliation and for-profit/non-profit status; all rates are subject to change):

SortDifferentiationINTERNAL BASE RATES FOR HARVARD AND HMS FACULTY\*HSCI AFFILIATESEXTERNAL NON-PROFIT RESEARCHEXTERNAL COMMERCIAL (FOR-PROFIT)Module 1: Skeletal Muscle Differentiation (1 sample)

$1,781



$2,048



$2,547



$3,562







*\*"Harvard and HMS Faculty" includes two groups of users: 1) all Harvard faculty and researchers using a Harvard University 33-digit billing code, and 2) HMS Quad A and Quad B faculty members.*

## Service Details

### Differentiation Module 1: hPSC differentiation into skeletal muscle

In this module, which takes about **8-12 weeks** to complete, the iPS Core will differentiate a human pluripotent stem cell (hPSC) line into functional myoblasts.

Service steps include an initial thawing/passaging of the hPSC line of interest, 30 days of stepwise differentiation, filtration of early myoblasts, and a final expansion.

This module delivers a cryopreserved stock of the early myoblasts (at p0) and a cryopreserved stock of expanded myoblasts (at p1). The expanded myoblasts are optimal for secondary differentiation into myofibers.



 

    ![schematic of the information in skeletal muscle service description](/sites/g/files/omnuum10036/files/styles/hwp_1_1__720x720_scale/public/2026-04/SkM%20Differentiation%20Service.png?itok=FN-4wk6G)